Control and destruction of microbesmethods of control a) Principles and methods of microbialand destruction of controlmicrobes -Sterilization-Disinfection the CSSD-Chemotherapy and antibiotics-Pasteurizationb) Medical and surgical asepsisc) Bio-safety and waste management Definitions (1) Sterile :-Free from micro-organism of any kind. (2) Sterilization:– Process of killing or removing microbes including pathogenic, non-pathogenic, spores. (3) Disinfection :- It is the process of removing or killing pathogenic organisms which does not necessarily include non-pathogenic organisms.